![]() The Invitrogen pGlow and pBlue TOPO TA Expression Kits provide the convenience of TOPO cloning of your PCR products, and the option to quantify promoter activity with either Cycle 3 GFP (pGlow) or b - galactosidase (pBlue) expression. If you’re trying to characterize an unknown promoter, you’ll need a cloning and reporter system to monitor expression efficiencies from your PCR-generated mutants. These pTracer/Bsd Vectors express the zeocin resistance gene as a fusion product with the Cycle 3 GFP. The Invitrogen pTracer SV40 and CMV vectors are for expression of your protein without any epitope tags. The pTracer EF vector will allow expression of your protein with carboxy-terminal V-5 and 6xHis epitope tags. These Invitrogen pTracer/Bsd Vectors express the blasticidin resistance gene as a fusion product with the Cycle 3 GFP.Ĭhoose from SV40, CMV or EF-1 a promoters for expressing your gene of interest. The Invitrogen pTracer CMV vector is for expression of your protein without any epitope tags. The Invitrogen pTracer EF vector will allow expression of your protein with carboxy-terminal V-5 and 6xHis epitope tags. Although nearly as bright as the Vivid Colors FPs, you’ll need to use the proper filter set to observe the fully-enhanced brightness.Ĭhoose from CMV or EF-1 a promoters for expressing your gene of interest. These vectors express the Cycle 3 Green Fluorescent Protein (Cycle 3 GFP) which has spectral excitation and emission wavelengths identical to wild-type GFP. To express your gene with alternative promoters, or with other selectable markers for stable-cell generation, consider the Invitrogen pTracer vectors series. Learn more about viral delivery of mammalian expression vectors. This vector allows you to generate high-titer Lentiviral preps that express EmGFP and provides for blasticidin selection. To optimize lentiviral packaging and transduction efficiencies and monitor infected cells, you need the Invitrogen Vivid Colors pLenti 6.2/EmGFP-GW Expression Control Vector. Lentiviral (Vivid Color) emerald GFP control expression vector.Configured for bacterial expression and fluorescent protein purification, these vectors can also be used for subcloning to construct customized mammalian expression vectors. These proteins are commonly used with the YFP and EmGFP for multi-color applications. The pRSET/CFP (cyan) and pRSET/BFP (blue) vectors contain alternative color versions of fluorescent proteins derived from Aequorea victoria. Create your own vectors with other Vivid Colors fluorescent proteins.If you already have a clone of your protein, you can amplify it by PCR and clone it with TOPO technology to prepare the entry-clone. The Ultimate ORF collection is a comprehensive and convenient source of full-length entry clones for this purpose. You’ll need to use a Gateway entry-clone version of your gene to clone into this destination (DEST) vector. The Invitrogen Vivid Colors pcDNA 6.2/ EmGFP-Bsd / V5-DEST vector allows you to rapidly identify transfected cells to look for biological activity conferred by your gene. Co-expression of your protein with a Vivid Colors fluorescent proteinĮxpress your protein in its native-protein configuration AND EmGFP from a single vector.Regardless of the cloning techniques you select, the Invitrogen Ultimate ORF collection is a comprehensive and convenient source of full-length cDNA clones for creating fusion proteins. Invitrogen Gateway Destination Vectors require the use of an entry clone, while the TOPO vectors require only a PCR product of your GOI (Invitrogen TA Cloning). To create a Vivid Colors fusion with protein, you can employ either Gateway or TOPO cloning technology. Emerald Green (EmGFP) and Yellow (YFP) Fluorescent Protein versions are available. These Invitrogen Vivid Colors vectors are for generation of N-terminal or C-terminal fluorescent protein fusions to your gene-of-interest (GOI).
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